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slide seq  (TaKaRa)


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    Structured Review

    TaKaRa slide seq
    Slide Seq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slide+seq/Seeker+Spatial+Transcriptomics+Kit/pmc13017465-3-0-1
    Average 95 stars, based on 157 article reviews
    slide seq - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    other:

    Article Title: stPipe: a flexible and streamlined R/Bioconductor pipeline for preprocessing sequencing-based spatial transcriptomics data
    Article Snippet: For Stereo-seq, Slide-seq, and Curio-seeker, barcodes are randomly distributed across spatial coordinates which must be mapped in a sample-specific way.

    Generated:

    Article Title: stPipe: a flexible and streamlined R/Bioconductor pipeline for preprocessing sequencing-based spatial transcriptomics data
    Article Snippet: .. stPipe is an R package that can handle data generated from popular sST protocols, including 10× Visium, BGI Stereo-seq, Slide-seq, and Curio-seeker (Fig. ). ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: Exploring the human brain: spatial transcriptomics challenges and approaches in post-mortem analysis.
    Article Snippet: .. Slide-seq (Curio Seeker) FF 10 μm 3 x 3 mm OR 10 x 10 mm Kamath et al.17 Untargeted - whole transcriptome Low Slide-seq V2 FF 10 μm 0.3 mm diameter Untargeted - whole transcriptome Moderate to High Greater capture efficiency and sensitivity than slide-seq FFPE = formalin fixed paraffin embedded; FF = fresh frozen. ..

    Sequencing:

    Article Title: stPipe: a flexible and streamlined R/Bioconductor pipeline for preprocessing sequencing-based spatial transcriptomics data
    Article Snippet: .. Other platform-specific information such as the spatial location csv file path for Slide-seq or Curio-seeker data, specific fa and gff files for polyA-based sequencing methods, tiff image path for pixel computation, path to h5 mapping file for Stereo-seq data, the QC method, and the ratio set for Run_QC function. ..



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    TaKaRa slide seq spatial transcriptomics experiment
    AIR-SPACE enables the mapping of adaptive immune receptor (AIR) clonotypes and <t>transcriptomics</t> in situ. ( A ) Schematic of the experimental design and methodology, including the generation of long-read (LR) and short-read (SR). ( B ) Spatial mapping of cell types across the LN sections at different time points postinfection. (Scale bar, 500 μm.) ( C ) Spatial mapping of AIR clonotypes across the LN sections, with immunoglobulin (IG) clones shown in blue and T cell receptor (TCR) clones shown in red; outlined with germinal center (GC) regions in LNs from D10PI to D21PI. ( D ) Multiplexed RNA FISH staining for T cell marker Trbc2 (green), B cell marker Ms4a1 (red), and DAPI (blue) across all samples on sister sections. (Scale bar 500 μm.)
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    Image Search Results


    AIR-SPACE enables the mapping of adaptive immune receptor (AIR) clonotypes and transcriptomics in situ. ( A ) Schematic of the experimental design and methodology, including the generation of long-read (LR) and short-read (SR). ( B ) Spatial mapping of cell types across the LN sections at different time points postinfection. (Scale bar, 500 μm.) ( C ) Spatial mapping of AIR clonotypes across the LN sections, with immunoglobulin (IG) clones shown in blue and T cell receptor (TCR) clones shown in red; outlined with germinal center (GC) regions in LNs from D10PI to D21PI. ( D ) Multiplexed RNA FISH staining for T cell marker Trbc2 (green), B cell marker Ms4a1 (red), and DAPI (blue) across all samples on sister sections. (Scale bar 500 μm.)

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection

    doi: 10.1073/pnas.2504742123

    Figure Lengend Snippet: AIR-SPACE enables the mapping of adaptive immune receptor (AIR) clonotypes and transcriptomics in situ. ( A ) Schematic of the experimental design and methodology, including the generation of long-read (LR) and short-read (SR). ( B ) Spatial mapping of cell types across the LN sections at different time points postinfection. (Scale bar, 500 μm.) ( C ) Spatial mapping of AIR clonotypes across the LN sections, with immunoglobulin (IG) clones shown in blue and T cell receptor (TCR) clones shown in red; outlined with germinal center (GC) regions in LNs from D10PI to D21PI. ( D ) Multiplexed RNA FISH staining for T cell marker Trbc2 (green), B cell marker Ms4a1 (red), and DAPI (blue) across all samples on sister sections. (Scale bar 500 μm.)

    Article Snippet: Slide-seq spatial transcriptomics experiment was performed using the Curio Seeker Kit (Curio Bioscience) according to manufacturer instructions.

    Techniques: In Situ, Clone Assay, Staining, Marker